Helicases are responsible for the unwinding of double-stranded DNA to produce single-stranded DNA...
Category - Molecular Biology
How to Design Primers for QPCR
Quantitative or real-time PCR is used as a routine assay for the monitoring the relative changes in...
Why are Viruses Used in Gene Therapy
Gene therapy is the introduction of foreign genetic material into body cells to compensate for...
How is a Genomic Library Produced
A genomic library is one of the two types of DNA libraries that contain total chromosomal DNA of a...
How to Isolate mRNA from Total RNA
The oligo-dT/carrier combinations are mainly used in the purification of mRNA from total RNA by a...
How Does Western Blotting Work
Western blotting is a technique in molecular biology used in the detection of a specific protein...
How Does Southern Blotting Work
Southern blotting is a technique used for the detection of specific DNA fragment within a sample...
How to Read a Plasmid Map
A plasmid map is the relative positions of the elements within the plasmid. A plasmid map can be...
How to Calculate Transfection Efficiency
Transfection efficiency can be calculated by counting the number of cells transfected over the...
How Does DNA Sequencing Work
Sequencing is the process involved in the determination of a nucleotide sequence of a particular...